123,123

內(nèi)容簡(jiǎn)介

　　植物生理學(xué)是研究植物生命活動(dòng)規(guī)律、揭示植物生命現(xiàn)象本質(zhì)的一門(mén)學(xué)科。植物生理學(xué)是農(nóng)學(xué)、園藝、植物保護(hù)、種子科學(xué)與工程、設(shè)施農(nóng)業(yè)科學(xué)與工程、植物科學(xué)與技術(shù)、園林等涉農(nóng)專(zhuān)業(yè)的專(zhuān)業(yè)基礎(chǔ)課程。植物生理學(xué)實(shí)驗(yàn)是該課程重要的實(shí)踐性教學(xué)環(huán)節(jié)，通過(guò)實(shí)驗(yàn)操作能進(jìn)一步加深學(xué)生對(duì)理論知識(shí)的理解，提高學(xué)生動(dòng)手實(shí)踐能力，將理論和實(shí)踐有機(jī)結(jié)合，為解決農(nóng)業(yè)生產(chǎn)實(shí)際問(wèn)題奠定良好的基礎(chǔ)。《植物生理學(xué)實(shí)驗(yàn)實(shí)訓(xùn)綜合教程》主要包括植物種子的萌發(fā)、水分代謝、礦質(zhì)元素缺乏對(duì)植物生命活動(dòng)的影響、植物光合性能的評(píng)價(jià)、植物碳氮代謝、植物生長(zhǎng)物質(zhì)的生理效應(yīng)及其對(duì)植物生長(zhǎng)發(fā)育的影響、植物組織培養(yǎng)、植物逆境生理等內(nèi)容，一共9章。本書(shū)基本上涵蓋了植物生理學(xué)所有的章節(jié)內(nèi)容，且充分考慮各章節(jié)的重點(diǎn)、難點(diǎn)，為我們進(jìn)一步理解和鞏固相關(guān)理論知識(shí)提供了幫助。本書(shū)非常注重理論教學(xué)和實(shí)踐應(yīng)用的深度結(jié)合，由濰坊學(xué)院、山東農(nóng)業(yè)工程學(xué)院、濰坊職業(yè)學(xué)院和山東省農(nóng)業(yè)技術(shù)推廣中心4家單位合作完成，既有本科高校和高職院校的教師，也有從事農(nóng)業(yè)技術(shù)推廣的一線工作人員，他們從不同的專(zhuān)業(yè)角度來(lái)合理安排實(shí)驗(yàn)內(nèi)容，通過(guò)簡(jiǎn)單易做的植物實(shí)驗(yàn)來(lái)驗(yàn)證復(fù)雜微觀的科學(xué)知識(shí)，啟發(fā)大家去探索植物生長(zhǎng)的內(nèi)在規(guī)律，以此提高作物生產(chǎn)潛力。

作者簡(jiǎn)介

暫缺《植物生理學(xué)實(shí)驗(yàn)實(shí)訓(xùn)綜合教程（中英雙語(yǔ)版）》作者簡(jiǎn)介

圖書(shū)目錄

中文篇

第1章植物生理學(xué)實(shí)驗(yàn)基礎(chǔ) 3
第2章植物種子的萌發(fā) 14
第3章植物水分代謝 22
第4章礦質(zhì)元素缺乏對(duì)植物生命活動(dòng)的影響 30
第5章植物光合性能的評(píng)價(jià) 42
第6章植物碳氮代謝 59
第7章植物生長(zhǎng)物質(zhì)的生理效應(yīng)及其對(duì)植物生長(zhǎng)發(fā)育的影響 72
第8章植物組織培養(yǎng) 83
第9章植物逆境生理 95

附　錄 115
附錄1 相關(guān)理論知識(shí)鞏固與思考115
附錄2 化學(xué)品標(biāo)志符號(hào)202
附錄3 常用酸堿的濃度203
附錄4 常用固態(tài)酸、堿、鹽的物質(zhì)的量濃度配制參考 203
附錄5 常用緩沖液的配制204
附錄6 常用酸堿指示劑209
附錄7 標(biāo)準(zhǔn)計(jì)量單位210
附錄8 常用植物生長(zhǎng)物質(zhì)的一些化學(xué)特性212
參考文獻(xiàn) 213

英文篇
Chapter 1 Experimental Basis of Plant Physiology 217

11 Plant physiology laboratory rules217

12 Classification and preparation methods of chemical reagent218

13 Collection, processing and preservation of experimental material220

14 Basic methods of experimental studies on plant physiology228

Chapter 2 Plant Seed Germination 232

21　 Determination of seed germination rate, germination potential and
germination index233

22　 Rapid determination of seed viability234

23　 Determination of seed vigor236

24　 The formation and determination of the amylase activity during seed
germination238

25　 Changes in fatty acid content of oil seeds during germination239

26　 Changes in amino acid content during seed germination241

Chapter 3　 Plant Water Metabolism 243

31　 Determination of plant leaf water content, relative water content and
water saturation deficit244

32　 Determination of free and bound water content in plant leaves
(Malincic method) 245

33　 Determination of plant tissue water potential (Small flow method) 247

34　 Determination of plant tissue osmotic potential (Plasma wall
separation method) 249

35　 Determination of transpiration rate251

Chapter 4　 Effects of Mineral Element Deficiency on Plant Life
Activities 254

41　 Element deficiency symptoms in plants (solution culture) 255

42　 Determination of nitrate reductase activity259

43　 Determination of root activity (α - naphthylamine oxidation method) 261

44　 Determination of nitrate nitrogen in plants263

45　 Determination of plant phosphorus (molybdenum blue method) 265

Chapter 5　 Evaluation of Plant Photosynthetic Performance 268

51　 Extraction and physicochemical properties of photosynthetic pigments269

52　 Separation of photosynthetic pigments and determination of absorption
spectra271

53　 Determination of chloroplast pigment content273

54　 Determination of plant leaf photosynthetic rate and gas exchange parameters
276

55　 Determination of chlorophyll fluorescence parameters280

56　 Determination of ribulose diphosphate carboxylase (RuBPCase)
activity286

57　 Determination of phosphoenolpyruvate carboxylase (PEPCase)
activity289

Chapter 6　 Plant Carbon and Nitrogen Metabolism 291

61　 Determination of sucrose, glucose and fructose content292

62　 Determination of free amino acid content294

63　 Determination of sucrose synthase and sucrose phosphatase activities296

64　 Determination of α - amylase and β - amylase activities298

65　 Determination of glutamine synthetase activity302

66　 Determination of soluble protein content304

Chapter 7　 The Physiological Effects of Plant Growth Substances and
Their Effects on Plant Development 307

71　 Effects of auxin on the growth of wheat root and shoot308

72　 Effects of gibberellin on the growth of wheat seedlings310

73　 Effect of ethephon on the fruit ripening310

74　 Abscissive effects of abscisic acid (ABA) on plant petioles311

75　 Effects of gibberellin (GA3) and abscisic acid (ABA) on seed
germination313

76　 Synthesis of α - amylase in seed induced by gibberellin (GA3) 314

77　 Effects of abscisic acid (ABA) on stomatal movement317

78　 Determination of phytohormones by liquid chromatography318

Chapter 8　 Plant Tissue Culture 320

81　 Preparation of culture medium321

82　 Sterilization, disinfection and inoculation325

83　 Subculture and propagation330

84　 Acclimation, transplanting and management of tube seedlings334

Chapter 9　 Plant Stress Physiology 336

91　 Determination of proline content337

92　 Determination of total flavonoid content339

93　 Determination of ascorbic acid (AsA, AA) 341

94　 Determination of glutathione (GsH) 345

95　 Determination of cytoplasmic membrane permeability - conductance
method347

96　 Identification of degree of membrance peroxidation - determination of
malondialdehyde content (MDA)349

97　 Identification of cell death in plant tissues (Trypan blue staining method) 351

98　 Determination of hydrogen peroxide (H2O2) content352

99　 Determination of superoxide anion production rate354

910　 Determination of superoxide dismutase (SOD) activity356

911　 Determination of peroxidase (POD) activity358

912　 Determination of catalase (CAT) activity360